plx304 targ1 wt Search Results


plx304  (Addgene inc)
95
Addgene inc plx304
Plx304, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plx304+targ1+wt/pLX304+(Plasmid+%2325890)/pmc08501950-82-35-42
Average 95 stars, based on 1 article reviews
plx304 - by Bioz Stars, 2026-09
95/100 stars
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pet28a  (Addgene inc)
92
Addgene inc pet28a
TARG1 removes thymidine-linked ADP-ribose from DNA and confers resistance to DarT toxicity in bacteria. ( A ) Structural comparison between TaqDarG-macrodomain (orange) bound to ADP-ribose (red) and TARG1 (light blue) with a covalent lysyl-ADP-ribose linkage (dark blue). To the right is a detailed view of the DarG catalytic lysine 80 (orange sticks) and TARG1 catalytic lysine 84 (blue sticks). ( B ) UV detection of DarT ADP-ribosylated DNA oligonucleotide de-ADP-ribosylation reactions with TaqDarG-macrodomain and TARG1. ( C ) Bacterial DarT toxicity rescue assay in BL21 DE3 using pBAD DarT and pET encoding DarG-macrodomain WT, DarG-macrodomain <t>K80A,</t> TARG1 WT or TARG1 K84A. pBAD expression is controlled with glucose or arabinose and pET expression is controlled with IPTG.
Pet28a, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plx304+targ1+wt/pET28a(%2B)+6xHis-TEV-IFIT1+(Plasmid+%2353557)/pmc08501950-82-9-42
Average 92 stars, based on 1 article reviews
pet28a - by Bioz Stars, 2026-09
92/100 stars
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pbad33 v5 dart  (Addgene inc)
93
Addgene inc pbad33 v5 dart
TARG1 removes thymidine-linked ADP-ribose from DNA and confers resistance to DarT toxicity in bacteria. ( A ) Structural comparison between TaqDarG-macrodomain (orange) bound to ADP-ribose (red) and TARG1 (light blue) with a covalent lysyl-ADP-ribose linkage (dark blue). To the right is a detailed view of the DarG catalytic lysine 80 (orange sticks) and TARG1 catalytic lysine 84 (blue sticks). ( B ) UV detection of DarT ADP-ribosylated DNA oligonucleotide de-ADP-ribosylation reactions with TaqDarG-macrodomain and TARG1. ( C ) Bacterial DarT toxicity rescue assay in BL21 DE3 using pBAD DarT and pET encoding DarG-macrodomain WT, DarG-macrodomain <t>K80A,</t> TARG1 WT or TARG1 K84A. pBAD expression is controlled with glucose or arabinose and pET expression is controlled with IPTG.
Pbad33 V5 Dart, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plx304+targ1+wt/pBAD33+(Plasmid+%2310686)/pmc08501950-82-4-42
Average 93 stars, based on 1 article reviews
pbad33 v5 dart - by Bioz Stars, 2026-09
93/100 stars
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plix 403  (Addgene inc)
96
Addgene inc plix 403
TARG1 removes thymidine-linked ADP-ribose from DNA and confers resistance to DarT toxicity in bacteria. ( A ) Structural comparison between TaqDarG-macrodomain (orange) bound to ADP-ribose (red) and TARG1 (light blue) with a covalent lysyl-ADP-ribose linkage (dark blue). To the right is a detailed view of the DarG catalytic lysine 80 (orange sticks) and TARG1 catalytic lysine 84 (blue sticks). ( B ) UV detection of DarT ADP-ribosylated DNA oligonucleotide de-ADP-ribosylation reactions with TaqDarG-macrodomain and TARG1. ( C ) Bacterial DarT toxicity rescue assay in BL21 DE3 using pBAD DarT and pET encoding DarG-macrodomain WT, DarG-macrodomain <t>K80A,</t> TARG1 WT or TARG1 K84A. pBAD expression is controlled with glucose or arabinose and pET expression is controlled with IPTG.
Plix 403, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plx304+targ1+wt/pLIX_403+(Plasmid+%2341395)/pmc08501950-82-33-42
Average 96 stars, based on 1 article reviews
plix 403 - by Bioz Stars, 2026-09
96/100 stars
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Image Search Results


TARG1 removes thymidine-linked ADP-ribose from DNA and confers resistance to DarT toxicity in bacteria. ( A ) Structural comparison between TaqDarG-macrodomain (orange) bound to ADP-ribose (red) and TARG1 (light blue) with a covalent lysyl-ADP-ribose linkage (dark blue). To the right is a detailed view of the DarG catalytic lysine 80 (orange sticks) and TARG1 catalytic lysine 84 (blue sticks). ( B ) UV detection of DarT ADP-ribosylated DNA oligonucleotide de-ADP-ribosylation reactions with TaqDarG-macrodomain and TARG1. ( C ) Bacterial DarT toxicity rescue assay in BL21 DE3 using pBAD DarT and pET encoding DarG-macrodomain WT, DarG-macrodomain K80A, TARG1 WT or TARG1 K84A. pBAD expression is controlled with glucose or arabinose and pET expression is controlled with IPTG.

Journal: Nucleic Acids Research

Article Title: TARG1 protects against toxic DNA ADP-ribosylation

doi: 10.1093/nar/gkab771

Figure Lengend Snippet: TARG1 removes thymidine-linked ADP-ribose from DNA and confers resistance to DarT toxicity in bacteria. ( A ) Structural comparison between TaqDarG-macrodomain (orange) bound to ADP-ribose (red) and TARG1 (light blue) with a covalent lysyl-ADP-ribose linkage (dark blue). To the right is a detailed view of the DarG catalytic lysine 80 (orange sticks) and TARG1 catalytic lysine 84 (blue sticks). ( B ) UV detection of DarT ADP-ribosylated DNA oligonucleotide de-ADP-ribosylation reactions with TaqDarG-macrodomain and TARG1. ( C ) Bacterial DarT toxicity rescue assay in BL21 DE3 using pBAD DarT and pET encoding DarG-macrodomain WT, DarG-macrodomain K80A, TARG1 WT or TARG1 K84A. pBAD expression is controlled with glucose or arabinose and pET expression is controlled with IPTG.

Article Snippet: Plasmids used here include: pBAD33-V5-DarT ( Thermus aquaticus ), pET28a-6xHis-TARG1-WT, pET28a-6xHis-TARG1-K84A, pET28a-6xHis-DarG-WT-1–155aa ( Thermus aquaticus ), pET28a-6xHis-DarG-K80A-1–155aa ( Thermus aquaticus ), pLIX_403-GFP-DarT-WT ( Thermus aquaticus ), pLIX_403-GFP-DarT-E160A ( Thermus aquaticus ), pLX304-TARG1-WT, pLX304-TARG1-K84A. pLIX_403 and pLX304 were gifts from David Root (pLIX_403 Addgene: #41395, pLX304 Addgene: #25890)

Techniques: Bacteria, Comparison, Rescue Assay, Expressing